ASIS International (ASIS) Professional Certified Investigator Practice Exam

Which of the following characteristics does RFLP provide in genetic analysis?

1. Only eliminates possible genetic matches

2. Positively identifies genetic typology

3. Chemically replicates DNA

4. Provides a general analysis of DNA segments

The correct answer is: Positively identifies genetic typology

RFLP, or Restriction Fragment Length Polymorphism, is a technique used in genetic analysis that focuses on variations in DNA sequences. The method involves cutting DNA samples with specific enzymes, called restriction enzymes, which create fragments that vary in length depending on the underlying genetic variations among individuals. The characteristic of positively identifying genetic typology through RFLP testing stems from its ability to create a specific pattern of DNA fragments that can be compared between individuals. This pattern reflects the unique genetic makeup of an individual, allowing for clear distinctions to be made regarding genetic relationships and potential matches. This capability is particularly useful in applications such as paternity testing and forensic investigations. In contrast, the other options provided do not accurately reflect the functionality of RFLP. Options discussing elimination of genetic matches or general analysis of DNA segments do not capture the specificity and identification power of the RFLP method. Additionally, claiming that RFLP chemically replicates DNA misrepresents the technique, as RFLP focuses on analysis rather than replication of genetic material. Overall, the strength of RFLP lies in its precision in identifying unique genetic profiles.